R-Spondin 1

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Cat. No.	Species	Product Description	Structure	Purity	Feature
RS1-H5269	Human	Human R-Spondin 1 / RSPO1 (21-146) Protein, Fc Tag
RS6-H4220	Human	Human R-Spondin 1 / RSPO1 (21-146) Protein, His Tag, premium grade
RS1-H4221	Human	Human R-Spondin 1 / RSPO1 Protein, His Tag

Part of Bioactivity data

RS1-H5269-Cell-based assay

Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) induced TCF reporter activity in HEK293 cells. The EC50 for this effect is 0.0311 µg/mL (Routinely tested).

Protocol

RS6-H4220-Cell-based assay

Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220) stimulates proliferation of TCF/LEF (Luc) HEK293 Reporter Cell. The specific activity of Human R-Spondin 1 (21-146), His Tag, premium grade is >1.00 X 10^4 U/mg (QC tested).

Protocol

RS6-H4220-ELISA

Immobilized Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 0.01-0.156 μg/mL (QC tested).

Protocol

RS1-H5269-ELISA

Immobilized Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) at 5 μg/mL (100 μL/well) can bind Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) with a linear range of 0.008-0.5 μg/mL (QC tested).

Protocol

to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/¦Â-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for ¦Â-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus. Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively. R-Spondin-1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. Human RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY. R-Spondin-1 regulates Wnt/¦Â-catenin by competing with the Wnt antagonist DKK1 for binding to the Wnt co receptors, Kremen and LRP6, reducing their DKK1 mediated internalization. Reports differ on whether R-spondin 1 binds LRP6 directly.

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